R. flavovittata, categorized as “data deficient” (DD) on the Red List, inhabits the leaf litter vegetation up to 1.2 m above the ground in lowland primary forests and can be found in the mouth of Río Tahuayo on the northern bank at 120 m a.s.l., and in the Amazon Basin in Departamento Loreto, Peru.

The frogs reach the size up to 17 mm, with the males only slightly smaller as the females. Ground color of the dorsal side and flanks varies from deep brown to black. Some irregular, yellow to light orange-colored spots on the head can be seen and there is a wide median and two dorsolateral stripes, sometimes broken, in the same color. Furthermore, a wide supralabial stripe, from the tip of the snout to the armpit, extends onto the adjacent areas of the upper arm, and then continuous as an often discontinuing ventrolateral stripe towards the groin. On the upper side of the thigh near the groin, there is a larger, equally yellow to light orange-colored spot. Limbs are marked with irregularly large, oft merged, dark brown to black dots.

The breeding of this species resembles that of the R. reticulata and R. amazonica.